論文
【カルボキシル化ポリリジン「不凍ポリアミノ酸」】
◆細胞保存
1. Long-term cryopreservation of
human mesenchymal stem cells using carboxylated poly-l-lysine without the addition of proteins or dimethyl sulfoxide. J Biomater Sci Polym Ed. 2013
Aug;24(12):1484-97. doi: 10.1080/09205063.2013.771318. Epub 2013 Feb 22.
2. Effective vitrification of human induced pluripotent stem cells using carboxylated ε-poly-l-lysine. Cryobiology. 2011 Oct;63(2):76-83. doi:
10.1016/j.cryobiol.2011.05.003. Epub 2011 May 20.
3. Polyampholytes as cryoprotective
agents for mammalian cell cryopreservation. 2010;19(6):691-9. doi: 10.3727/096368910X508780. Epub 2010 Jun 3.
4. Polyampholytes as low toxic
efficient cryoprotective agents with antifreeze protein properties. Biomaterials. 2009 Sep;30(27):4842-9. doi: 10.1016/j.biomaterials.2009.05.025. Epub
2009 Jun 9.
5. Cryopreservation of bovine somatic cells using antifreeze polyamino-acid (carboxylated poly-l-lysine). Cryobiology. 2017 Feb 1. pii: S0011-2240(16)30436-9. doi: 10.1016/j.cryobiol.2017.01.010. NEW!!
◆軟骨細胞シート保存
1. Development of a novel
vitrification method for chondrocyte sheets. BMC Biotechnol. 2013 Jul 25;13(1):58. [Epub ahead of print]
◆スキャフォールド
1. Cell encapsulation and
cryostorage in PVA-gelatin cryogels: incorporation of carboxylated ε-poly-L-lysine as cryoprotectant. J Tissue Eng Regen Med. 2012 Apr;6(4):280-90. doi:
10.1002/term.431. Epub 2011 Jun 27.
◆受精卵保存(不妊治療)
1. Successful vitrification of pronuclear-stage pig embryos with a novel cryoprotective agent, carboxylated ε-poly-L-lysine. PLoS One. 2017 Apr 27;12(4):e0176711. doi: 10.1371/journal.pone.0176711. eCollection 2017. NEW!!
2. The Beneficial Effect of Carboxylated Poly-L-Lysine on Cryosurvival of Vitrified Early Stage. Cryo Letters. 2017 Jan/Feb;38(1):1-6. NEW!!
3. Development of a Low Toxicity and Completely Serum-Free Vitrification System Combining a Cryo-Nano-Hole Vitrification Container with Antifreeze Polyamino-acid (Carboxylated Poly-L-Lysine) for assisted reproductive technology (ART). J. Reprod. Engineer. 2011; Vol. 14 No. 2: 13-36.
◆凍結機序
1. Antifreeze effect of carboxylated ε-poly-L-lysine on the growth kinetics of ice crystals. J Phys Chem B. 2014 Aug 28;118(34):10240-9. doi: 10.1021/jp507697q. Epub 2014 Aug 18.
◆DDS
1. Protein cytoplasmic delivery using polyampholyte nanoparticles and freeze concentration. Biomaterials. 2014 Aug;35(24):6508-18. doi: 10.1016/j.biomaterials.2014.04.030. Epub 2014 May 10.
【緑茶由来ポリフェノール(EGCg)】
◆皮膚繊維芽細胞
1. Preventive effects of
epigallocatechin-3-O-gallate against replicative senescence associated with p53 acetylation in human dermal fibroblasts. Oxid Med Cell Longev.
2012;2012:850684. doi: 10.1155/2012/850684. Epub 2012 Nov 18.
2. Epigallocatechin-3-gallate regulates
cell growth, cell cycle and phosphorylated nuclear factor-κB in human dermal fibroblasts. Acta Pharmacol Sin. 2011 May;32(5):637-46. doi:
10.1038/aps.2011.17. Epub 2011 Apr 25.
3. In vitro antifungal activity of epigallocatechin 3-O-gallate against clinical
isolates of dermatophytes. Yonsei Med J. 2011 May;52(3):535-8. doi: 10.3349/ymj.2011.52.3.535.
◆皮膚組織
1. Long-term preservation of rat
skin tissue by epigallocatechin-3-o-gallate. Cell Transplant. 2009;18(5):505-12.
2. Green tea polyphenols affect skin
preservation in rats and improve the rate of skin grafts. Cell Transplant. 2008;17(1-2):203-9.
◆組織バンク(移植)
1. A non-frozen living tissue bank for allotransplantation using green tea
polyphenols. Yonsei Med J. 2004 Dec 31;45(6):1025-34. Review.
◆移植片対宿主病
1. Attenuation of murine graft-versus-host disease by a tea polyphenol. Cell Transplant. 2012;21(5):909-18. doi: 10.3727/096368911X623934. Epub 2012 Feb 13.
◆腎臓
1. Epigallocatechin-3-gallate
protects kidneys from ischemia reperfusion injury by HO-1 upregulation and inhibition of macrophage infiltration. Transpl Int. 2011 May;24(5):514-22. doi:
10.1111/j.1432-2277.2011.01224.x. Epub 2011 Feb 3.
2. Protection of rabbit kidney from
ischemia/reperfusion injury by green tea polyphenol pretreatment. Arch Pharm Res. 2007 Nov;30(11):1447-54.
3. Polyphenol, an extract of green
tea, increases culture recovery rates of isolated islets from nonhuman primate pancreata and marginal grade human pancreata. Cell Transplant.
2004;13(2):145-52.
◆末梢神経
1. Storage and allogeneic
transplantation of peripheral nerve using a green tea polyphenol solution in a canine model. J Brachial Plex Peripher Nerve Inj. 2010 Nov 28;5:17. doi:
10.1186/1749-7221-5-17.
2. Optimal conditions for peripheral
nerve storage in green tea polyphenol: an experimental study in animals. Biotechnol Lett. 2005 May;27(9):655-60.
3. Peripheral nerve allografts
stored in green tea polyphenol solution. Transplantation. 2005 Mar 27;79(6):688-95.
4. Successful storage of peripheral
nerve before transplantation using green tea polyphenol: an experimental study in rats. Exp Neurol. 2003 Dec;184(2):688-96.
◆血管平滑筋細胞
1. Selective inhibitory effect of
epigallocatechin-3-gallate on migration of vascular smooth muscle cells. Molecules. 2010 Nov 19;15(11):8488-500. doi:
10.3390/molecules15118488.
◆大動脈平滑筋細胞
1. Inhibitory effects of
epigallocatechin-3-O-gallate on serum-stimulated rat aortic smooth muscle cells via nuclear factor-kappaB down-modulation. Biochem Biophys Res Commun.
2006 Jun 23;345(1):148-55. Epub 2006 Apr 27.
◆ヒト伏在静脈
1. Long-term preservation of human
saphenous vein by green tea polyphenol under physiological conditions. Tissue Eng. 2005 Jul-Aug;11(7-8):1054-64.
2. Effects of green tea polyphenol
on preservation of human saphenous vein. J Biotechnol. 2004 May 27;110(2):109-17.
3. Preservation of human saphenous
vein against reactive oxygen species-induced oxidative stress by green tea polyphenol pretreatment. Artif Organs. 2003 Dec;27(12):1137-42.
◆ヒト繊維肉腫
1. Apoptosis of human fibrosarcoma HT-1080 cells by
epigallocatechin-3-O-gallate via induction of p53 and caspases as well as suppression of Bcl-2 and phosphorylated nuclear factor-κB. Apoptosis. 2011
Jan;16(1):75-85. doi: 10.1007/s10495-010-0548-y.
◆微小血管内皮細胞
1. Prevention of reactive oxygen
species-induced oxidative stress in human microvascular endothelial cells by green tea polyphenol. Toxicol Lett. 2005 Feb 15;155(2):269-75.
◆心臓
1. Oral pretreatment with a green
tea polyphenol for cardioprotection against ischemia-reperfusion injury in an isolated rat heart model. J Thorac Cardiovasc Surg. 2011 Feb;141(2):511-7.
doi: 10.1016/j.jtcvs.2010.04.016. Epub 2010 May 23.
2. A novel method of 'preparative'
myocardial protection using green tea polyphenol in oral uptake. Interact Cardiovasc Thorac Surg. 2004 Dec;3(4):612-5.
◆骨軟骨
1. Biological and biomechanical
evaluations of osteochondral allografts preserved in cold storage solution containing epigallocatechin gallate. Cell Transplant. 2010;19(6):681-9. doi:
10.3727/096368910X508771. Epub 2010 Jun 3.
◆関節軟骨
1. Nonfrozen preservation of
articular cartilage by epigallocatechin-3-gallate reversibly regulating cell cycle and NF-kappaB expression. Tissue Eng Part A. 2010 Feb;16(2):595-603.
doi: 10.1089/ten.TEA.2009.0484.
◆造骨細胞
1. Protection of osteoblastic cells
from freeze/thaw cycle-induced oxidative stress by green tea polyphenol. Biotechnol Lett. 2005 May;27(9):655-60.
◆骨芽細胞
1. Protective effects of green tea
polyphenol against reactive oxygen species-induced oxidative stress in cultured rat calvarial osteoblast. Cell Biol Toxicol. 2003
Oct;19(5):325-37.
◆血小板
1. Preservation of platelets by
adding epigallocatechin-3-o-gallate to platelet concentrates. Cell Transplant. 2009;18(5):521-8.
◆ヒト幹細胞
1. Effects of epigallocatechin
gallate on osteogenic capability of human mesenchymal stem cells after suspension in phosphate-buffered saline. Tissue Eng Part A. 2010 Jan;16(1):91-100.
doi: 10.1089/ten.TEA.2008.0636.
◆足根繊維芽細胞
1. Reversible regulation of cell
cycle-related genes by epigallocatechin gallate for hibernation of neonatal human tarsal fibroblasts. Cell Transplant. 2009;18(4):459-69. doi:
10.3727/096368909788809776.
◆ステント
1. Development of epigallocatechin
gallate-eluting polymeric stent and its physicochemical, biomechanical and biological evaluations. Biomed Mater. 2009 Aug;4(4):044104. doi:
10.1088/1748-6041/4/4/044104. Epub 2009 Jul 8.
2. The behavior of vascular smooth
muscle cells and platelets onto epigallocatechin gallate-releasing poly(l-lactide-co-epsilon-caprolactone) as stent-coating materials. Biomaterials. 2008
Mar;29(7):884-93. Epub 2007 Nov 26.
◆抗菌
1. Highly antibiotic-resistant
Acinetobacter baumannii clinical isolates are killed by the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG). Clin Microbiol Infect. 2009
Apr;15(4):341-6.
◆肝細胞
1. reservation of porcine
hepatocytes in three-dimensional bioreactor at room temperature using epigallocatechin-3-gallate. Tissue Eng Part C Methods. 2009 Sep;15(3):345-53. doi:
10.1089/ten.tec.2008.0483.
◆創傷被覆
1. Enhanced wound healing by an
epigallocatechin gallate-incorporated collagen sponge in diabetic mice. Wound Repair Regen. 2008 Sep-Oct;16(5):714-20. doi:
10.1111/j.1524-475X.2008.00422.x.
◆抗腫瘍
1. Enhanced antitumor activities of
(-)-epigallocatechin-3-O-gallate fatty acid monoester derivatives in vitro and in vivo. Biochem Biophys Res Commun. 2008 Dec 26;377(4):1118-22. doi:
10.1016/j.bbrc.2008.10.128. Epub 2008 Nov 5.
◆体内動態
1. Time-dependent intracellular
trafficking of FITC-conjugated epigallocatechin-3-O-gallate in L-929 cells. Bioorg Med Chem. 2008 Nov 15;16(22):9652-9. doi: 10.1016/j.bmc.2008.10.009.
Epub 2008 Oct 7.
◆哺乳類組織
1. Non-frozen preservation of
mammalian tissue using green tea polyphenolic compounds. Biomed Mater. 2006 Mar;1(1):R18-29. doi: 10.1088/1748-6041/1/1/R03. Epub 2006 Mar 21.
Review.
◆リンパ球培養
1. Tea polyphenol inhibits
allostimulation in mixed lymphocyte culture. Cell Transplant. 2007;16(1):75-83.
◆ラット大動脈組織移植
1. Preservation of rat aortic tissue
transplant with green tea polyphenols. Cell Transplant. 2006;15(10):881-3.
◆細胞成長抑制
1. Hibernation, reversible cell
growth inhibition by epigallocatechin-3-O-gallate. J Biotechnol. 2007 Jan 20;127(4):758-64. Epub 2006 Sep 22.
◆真菌性感染症
1. Antifungal susceptibility of
epigallocatechin 3-O-gallate (EGCg) on clinical isolates of pathogenic yeasts. Biochem Biophys Res Commun. 2006 Aug 25;347(2):401-5. Epub 2006 Jun
15.
◆虚血/再潅流障害
1. Effect of green tea extracted
polyphenol on ischemia/reperfusion injury after cold preservation of rat lung. Transplant Proc. 2003 Feb;35(1):138-9. No abstract available.
◆ラット腹部大動脈瘤
1. Prevention of abdominal aortic aneurysm progression by oral administration of green tea polyphenol in a rat model. J Vasc Surg. 2016 Jul 26. pii: S0741-5214(16)30419-0. doi: 10.1016/j.jvs.2016.06.003. NEW!!
お知らせ
News-
2022年8月9日
当社の事業が化学工業日報に掲載されました。
-
2022年6月29日
当社の事業が日経産業新聞に掲載されました。
-
2022年6月21日
当社の事業が日本経済新聞に掲載されました。
-
2022年6月21日
ニュースリリース:低温保存液に関するHeartseed株式会社との共同開発契約の締結(詳細は以下PDFをご覧ください)
-
2021年10月5日
ニュースリリース:新型コロナウイルス(SARS-CoV-2)の不活化効果を実証 (詳細は以下PDFをご覧ください)
-
2021年5月
CryoBovシリーズが日本経済新聞およびに日刊工業新聞に掲載されました。
-
2021年5月
ウシ受精卵(胚)凍結用CPLL溶液 CryoBov for Embryoの販売開始
-
2021年5月
ウシ精液凍結用CPLL溶液 CryoBov for Spermの販売開始
-
2021年5月
高純度EGCg含有 お口のエステにESTINY(エスティニー)の販売開始
-
2017年5月
新しく論文を掲載しました。
-
2013年2月
ヒト組換え体タンパク質
IBUKI(Human Basic Fibroblast Growth Factor Recombinant)の販売開始 -
2012年4月
組換え体ヒト血清リコンビナント
IBUKI(Human Serum Albumin Recombinant)の販売開始 -
2010年7月
霊長類 ES / iPS 細胞用ガラス化
凍結保存液ステムセルキープの販売開始 -
2009年4月
細胞用凍結保存液クライオスカーレス
DMSOフリーの販売開始 -
2006年10月
皮膚組織用冷蔵保存液セリオキープの販売開始