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細胞・組織保存液の販売・研究開発
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論文

【カルボキシル化ポリリジン「不凍ポリアミノ酸」】

◆細胞保存
1.    Long-term cryopreservation of human mesenchymal stem cells using carboxylated poly-l-lysine without the addition of proteins or dimethyl sulfoxide. J Biomater Sci Polym Ed. 2013 Aug;24(12):1484-97. doi: 10.1080/09205063.2013.771318. Epub 2013 Feb 22.
2.    Effective vitrification of human induced pluripotent stem cells using carboxylated ε-poly-l-lysine. Cryobiology. 2011 Oct;63(2):76-83. doi: 10.1016/j.cryobiol.2011.05.003. Epub 2011 May 20.
3.    Polyampholytes as cryoprotective agents for mammalian cell cryopreservation. 2010;19(6):691-9. doi: 10.3727/096368910X508780. Epub 2010 Jun 3.
4.    Polyampholytes as low toxic efficient cryoprotective agents with antifreeze protein properties. Biomaterials. 2009 Sep;30(27):4842-9. doi: 10.1016/j.biomaterials.2009.05.025. Epub 2009 Jun 9.

5.    Cryopreservation of bovine somatic cells using antifreeze polyamino-acid (carboxylated poly-l-lysine). Cryobiology. 2017 Feb 1. pii: S0011-2240(16)30436-9. doi: 10.1016/j.cryobiol.2017.01.010. NEW!!


◆軟骨細胞シート保存
1.    Development of a novel vitrification method for chondrocyte sheets. BMC Biotechnol. 2013 Jul 25;13(1):58. [Epub ahead of print]

◆スキャフォールド
1.    Cell encapsulation and cryostorage in PVA-gelatin cryogels: incorporation of carboxylated ε-poly-L-lysine as cryoprotectant. J Tissue Eng Regen Med. 2012 Apr;6(4):280-90. doi: 10.1002/term.431. Epub 2011 Jun 27.

◆受精卵保存(不妊治療)

1.      Successful vitrification of pronuclear-stage pig embryos with a novel cryoprotective agent, carboxylated ε-poly-L-lysine. PLoS One. 2017 Apr 27;12(4):e0176711. doi: 10.1371/journal.pone.0176711. eCollection 2017. NEW!!

 

2.      The Beneficial Effect of Carboxylated Poly-L-Lysine on Cryosurvival of Vitrified Early Stage. Cryo Letters. 2017 Jan/Feb;38(1):1-6. NEW!!

 

3.    Development of a Low Toxicity and Completely Serum-Free Vitrification System Combining a Cryo-Nano-Hole Vitrification Container with Antifreeze Polyamino-acid (Carboxylated Poly-L-Lysine) for assisted reproductive technology (ART). J. Reprod. Engineer. 2011; Vol. 14 No. 2: 13-36.

 

◆凍結機序

1.      Antifreeze effect of carboxylated ε-poly-L-lysine on the growth kinetics of ice crystals. J Phys Chem B. 2014 Aug 28;118(34):10240-9. doi: 10.1021/jp507697q. Epub 2014 Aug 18.

 

DDS

1.   Protein cytoplasmic delivery using polyampholyte nanoparticles and freeze concentration. Biomaterials. 2014 Aug;35(24):6508-18. doi: 10.1016/j.biomaterials.2014.04.030. Epub 2014 May 10.

 

【緑茶由来ポリフェノール(EGCg)】

◆皮膚繊維芽細胞
1.    Preventive effects of epigallocatechin-3-O-gallate against replicative senescence associated with p53 acetylation in human dermal fibroblasts. Oxid Med Cell Longev. 2012;2012:850684. doi: 10.1155/2012/850684. Epub 2012 Nov 18.
2.    Epigallocatechin-3-gallate regulates cell growth, cell cycle and phosphorylated nuclear factor-κB in human dermal fibroblasts. Acta Pharmacol Sin. 2011 May;32(5):637-46. doi: 10.1038/aps.2011.17. Epub 2011 Apr 25.
3.    In vitro antifungal activity of epigallocatechin 3-O-gallate against clinical isolates of dermatophytes.  Yonsei Med J. 2011 May;52(3):535-8. doi: 10.3349/ymj.2011.52.3.535.

◆皮膚組織
1.    Long-term preservation of rat skin tissue by epigallocatechin-3-o-gallate. Cell Transplant. 2009;18(5):505-12.
2.    Green tea polyphenols affect skin preservation in rats and improve the rate of skin grafts. Cell Transplant. 2008;17(1-2):203-9.

◆組織バンク(移植)
1.    A non-frozen living tissue bank for allotransplantation using green tea polyphenols. Yonsei Med J. 2004 Dec 31;45(6):1025-34. Review.

◆移植片対宿主病

1.    Attenuation of murine graft-versus-host disease by a tea polyphenol. Cell Transplant. 2012;21(5):909-18. doi: 10.3727/096368911X623934. Epub 2012 Feb 13.


◆腎臓
1.    Epigallocatechin-3-gallate protects kidneys from ischemia reperfusion injury by HO-1 upregulation and inhibition of macrophage infiltration. Transpl Int. 2011 May;24(5):514-22. doi: 10.1111/j.1432-2277.2011.01224.x. Epub 2011 Feb 3.
2.    Protection of rabbit kidney from ischemia/reperfusion injury by green tea polyphenol pretreatment. Arch Pharm Res. 2007 Nov;30(11):1447-54.
3.    Polyphenol, an extract of green tea, increases culture recovery rates of isolated islets from nonhuman primate pancreata and marginal grade human pancreata. Cell Transplant. 2004;13(2):145-52.

◆末梢神経
1.    Storage and allogeneic transplantation of peripheral nerve using a green tea polyphenol solution in a canine model. J Brachial Plex Peripher Nerve Inj. 2010 Nov 28;5:17. doi: 10.1186/1749-7221-5-17.
2.    Optimal conditions for peripheral nerve storage in green tea polyphenol: an experimental study in animals. Biotechnol Lett. 2005 May;27(9):655-60.
3.    Peripheral nerve allografts stored in green tea polyphenol solution. Transplantation. 2005 Mar 27;79(6):688-95.
4.    Successful storage of peripheral nerve before transplantation using green tea polyphenol: an experimental study in rats. Exp Neurol. 2003 Dec;184(2):688-96.

◆血管平滑筋細胞
1.    Selective inhibitory effect of epigallocatechin-3-gallate on migration of vascular smooth muscle cells. Molecules. 2010 Nov 19;15(11):8488-500. doi: 10.3390/molecules15118488.

◆大動脈平滑筋細胞
1.    Inhibitory effects of epigallocatechin-3-O-gallate on serum-stimulated rat aortic smooth muscle cells via nuclear factor-kappaB down-modulation. Biochem Biophys Res Commun. 2006 Jun 23;345(1):148-55. Epub 2006 Apr 27.

◆ヒト伏在静脈
1.    Long-term preservation of human saphenous vein by green tea polyphenol under physiological conditions. Tissue Eng. 2005 Jul-Aug;11(7-8):1054-64.
2.    Effects of green tea polyphenol on preservation of human saphenous vein. J Biotechnol. 2004 May 27;110(2):109-17.
3.    Preservation of human saphenous vein against reactive oxygen species-induced oxidative stress by green tea polyphenol pretreatment. Artif Organs. 2003 Dec;27(12):1137-42.

◆ヒト繊維肉腫
1.    Apoptosis of human fibrosarcoma HT-1080 cells by epigallocatechin-3-O-gallate via induction of p53 and caspases as well as suppression of Bcl-2 and phosphorylated nuclear factor-κB. Apoptosis. 2011 Jan;16(1):75-85. doi: 10.1007/s10495-010-0548-y.

◆微小血管内皮細胞
1.    Prevention of reactive oxygen species-induced oxidative stress in human microvascular endothelial cells by green tea polyphenol. Toxicol Lett. 2005 Feb 15;155(2):269-75.

◆心臓
1.    Oral pretreatment with a green tea polyphenol for cardioprotection against ischemia-reperfusion injury in an isolated rat heart model. J Thorac Cardiovasc Surg. 2011 Feb;141(2):511-7. doi: 10.1016/j.jtcvs.2010.04.016. Epub 2010 May 23.
2.    A novel method of 'preparative' myocardial protection using green tea polyphenol in oral uptake. Interact Cardiovasc Thorac Surg. 2004 Dec;3(4):612-5.

◆骨軟骨
1.    Biological and biomechanical evaluations of osteochondral allografts preserved in cold storage solution containing epigallocatechin gallate. Cell Transplant. 2010;19(6):681-9. doi: 10.3727/096368910X508771. Epub 2010 Jun 3.

◆関節軟骨
1.    Nonfrozen preservation of articular cartilage by epigallocatechin-3-gallate reversibly regulating cell cycle and NF-kappaB expression. Tissue Eng Part A. 2010 Feb;16(2):595-603. doi: 10.1089/ten.TEA.2009.0484.

◆造骨細胞
1.    Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol. Biotechnol Lett. 2005 May;27(9):655-60.

◆骨芽細胞
1.    Protective effects of green tea polyphenol against reactive oxygen species-induced oxidative stress in cultured rat calvarial osteoblast. Cell Biol Toxicol. 2003 Oct;19(5):325-37.

◆血小板
1.    Preservation of platelets by adding epigallocatechin-3-o-gallate to platelet concentrates. Cell Transplant. 2009;18(5):521-8.

◆ヒト幹細胞
1.    Effects of epigallocatechin gallate on osteogenic capability of human mesenchymal stem cells after suspension in phosphate-buffered saline. Tissue Eng Part A. 2010 Jan;16(1):91-100. doi: 10.1089/ten.TEA.2008.0636.

◆足根繊維芽細胞
1.    Reversible regulation of cell cycle-related genes by epigallocatechin gallate for hibernation of neonatal human tarsal fibroblasts. Cell Transplant. 2009;18(4):459-69. doi: 10.3727/096368909788809776.

◆ステント
1.    Development of epigallocatechin gallate-eluting polymeric stent and its physicochemical, biomechanical and biological evaluations. Biomed Mater. 2009 Aug;4(4):044104. doi: 10.1088/1748-6041/4/4/044104. Epub 2009 Jul 8.
2.    The behavior of vascular smooth muscle cells and platelets onto epigallocatechin gallate-releasing poly(l-lactide-co-epsilon-caprolactone) as stent-coating materials. Biomaterials. 2008 Mar;29(7):884-93. Epub 2007 Nov 26.

◆抗菌
1.    Highly antibiotic-resistant Acinetobacter baumannii clinical isolates are killed by the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG). Clin Microbiol Infect. 2009 Apr;15(4):341-6.

◆肝細胞
1.    reservation of porcine hepatocytes in three-dimensional bioreactor at room temperature using epigallocatechin-3-gallate. Tissue Eng Part C Methods. 2009 Sep;15(3):345-53. doi: 10.1089/ten.tec.2008.0483.

 

◆創傷被覆
1.    Enhanced wound healing by an epigallocatechin gallate-incorporated collagen sponge in diabetic mice. Wound Repair Regen. 2008 Sep-Oct;16(5):714-20. doi: 10.1111/j.1524-475X.2008.00422.x.

◆抗腫瘍
1.    Enhanced antitumor activities of (-)-epigallocatechin-3-O-gallate fatty acid monoester derivatives in vitro and in vivo. Biochem Biophys Res Commun. 2008 Dec 26;377(4):1118-22. doi: 10.1016/j.bbrc.2008.10.128. Epub 2008 Nov 5.

◆体内動態
1.    Time-dependent intracellular trafficking of FITC-conjugated epigallocatechin-3-O-gallate in L-929 cells. Bioorg Med Chem. 2008 Nov 15;16(22):9652-9. doi: 10.1016/j.bmc.2008.10.009. Epub 2008 Oct 7.

◆哺乳類組織
1.    Non-frozen preservation of mammalian tissue using green tea polyphenolic compounds. Biomed Mater. 2006 Mar;1(1):R18-29. doi: 10.1088/1748-6041/1/1/R03. Epub 2006 Mar 21. Review.

◆リンパ球培養
1.    Tea polyphenol inhibits allostimulation in mixed lymphocyte culture. Cell Transplant. 2007;16(1):75-83.

◆ラット大動脈組織移植
1.    Preservation of rat aortic tissue transplant with green tea polyphenols. Cell Transplant. 2006;15(10):881-3.

◆細胞成長抑制
1.    Hibernation, reversible cell growth inhibition by epigallocatechin-3-O-gallate. J Biotechnol. 2007 Jan 20;127(4):758-64. Epub 2006 Sep 22.

◆真菌性感染症
1.    Antifungal susceptibility of epigallocatechin 3-O-gallate (EGCg) on clinical isolates of pathogenic yeasts. Biochem Biophys Res Commun. 2006 Aug 25;347(2):401-5. Epub 2006 Jun 15.

◆虚血/再潅流障害
1.    Effect of green tea extracted polyphenol on ischemia/reperfusion injury after cold preservation of rat lung. Transplant Proc. 2003 Feb;35(1):138-9. No abstract available.

 

◆ラット腹部大動脈瘤

1.   Prevention of abdominal aortic aneurysm progression by oral administration of green tea polyphenol in a rat model. J Vasc Surg. 2016 Jul 26. pii: S0741-5214(16)30419-0. doi: 10.1016/j.jvs.2016.06.003. NEW!!

 

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News

  • 2006年10月

    皮膚組織用冷蔵保存液セリオキープの販売開始

  • 2009年4月

    細胞用凍結保存液クライオスカーレス
    DMSOフリーの販売開始

  • 2010年7月

    霊長類 ES / iPS 細胞用ガラス化
    凍結保存液ステムセルキープの販売開始

  • 2012年4月

    組換え体ヒト血清リコンビナント
    IBUKI(Human Serum Albumin Recombinant)の販売開始

  • 2013年2月

    ヒト組換え体タンパク質
    IBUKI(Human Basic Fibroblast Growth Factor Recombinant)の販売開始

  • 20175

    新しく論文を掲載しました。

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